Development and characterization of regulatory biomolecular circuits for plasmid copy numbers in synthetic biology

Development and characterization of regulatory biomolecular circuits for plasmid copy numbers in synthetic biology (Prof. Abhilash Patel, Electrical Engineering)

The advancement of synthetic biology relies heavily on plasmids for recombinant gene expression, yet this approach presents significant challenges. Plasmid copy numbers within host cells can vary considerably. Low-copy plasmids may exhibit instability, risking loss or structural changes, especially under selective pressure, which complicates their use in long-term applications. On the other hand, the high replication demands of high-copy plasmids can impose a metabolic burden, impairing cell growth and productivity. Additionally, environmental factors, such as temperature fluctuations, can affect plasmid copy numbers, adding further complexity to the control and reproducibility of synthetic biological systems.

This proposal aims to overcome these obstacles by developing control systems specifically designed to regulate plasmid copy numbers within host cells. By addressing these multifaceted challenges, we endeavor to enhance the reliability, efficiency, and safety of plasmid-based applications in synthetic biology.