Establishment of a CDSpectrometer Facility

Circular dichroism is the difference in the absorption of left-handed circularly polarized light (L-CPL) and right-handed circularly polarized light (R-CPL) and occurs when a molecule contains one or more chiral chromophores (light-absorbing groups). A circular dichroism signal can be positive or negative, depending on whether L-CPL is absorbed to a greater extent than R-CPL (CD signal positive) or to a lesser extent (CD signal negative). Circular dichroism = ΔA (λ) = A (λ) LCPL - A (λ) RCPL, where λ is the wavelength. Circular dichroism (CD) spectroscopy is a spectroscopic technique where the CD of molecules is measured in the visible and ultra-violet region of the electro-magnetic spectrum. CD spectroscopy is used extensively to study chiral molecules of all types and sizes, but it is in the study of large biological molecules where it finds its most important applications. A primary use is in analyzing the secondary structure or conformation of macromolecules, particularly proteins, and because secondary structure is sensitive to its environment, e.g. temperature or pH, circular dichroism can be used to observe how secondary structure changes with environmental conditions or on interaction with other molecules. Structural, kinetic and thermodynamic information about macromolecules can be derived from circular dichroism spectroscopy.

This instrument has the following features:

CD spectra measurement with wavelength
Time course CD signal measurement
Facility of variable temperature(-10ºC to 110ºC) by PTC-423S/15 Peltier effect
Quartz cells with path length 10mm, 5mm, 2mm, 1mm, 0.2mm, 0.1mm
Measurement wavelength range 163 nm to 900nm.
Stray Light less than 0.0003 % (200nm).
Wavelength repeatability ±0.05nm (163 to 250 nm) and ±0.1nm (250 to 500nm), and ±0.2nm (500 to 900nm).


Core lab no. CL107E, Department of Chemistry
IIT Kanpur


Prof. S P Rath

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