Leica Microscope


Optical microscopy is the oldest and most utilized method for microstructural evaluation of materials. Optical Microscopy uses the geometry and refractive indices of transparent optics to focus light and induce a magnification. The optical microscope (Leica DM2500 M) is established in the Western lab from a DST grant for the micro-fabrication lab.

Basic Principle:

The objective lens is, at its simplest, a very high powered magnifying glass i.e. a lens with a very short focal length. This is brought very close to the specimen being examined so that the light from the specimen comes to a focus about 160 mm inside the microscope tube. This creates an enlarged image of the subject. This image is inverted and can be seen by removing the eyepiece and placing a piece of tracing paper over the end of the tube. By carefully focusing a brightly lit specimen, a highly enlarged image can be seen. It is this real image that is viewed by the eyepiece lens that provides further enlargement.

Unique Features:

Fully integrated and fully automated x, y and z direction stage. Fluorescence bulb is also attached for biological applications. Upto 20X magnification.


Department of Chemical Engineering,
IIT Kanpur


Prof. Deepak Kunzru

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