Thermal Cycler (PCR Machine) |
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A basic PCR set up requires several components and reagents. These components include:
There are many companies making Thermocyclers such as BioRad, Thermo, Eppendorf, having the basic set-up with some specific features: The Eppendorf Mastercycler personal is a high quality, compact thermal cycler. In addition to its speed and precise block homogeneity, it offers first-rate operational flexibility. Its universal block with a 25-well format can accommodate 25 x 0.2 ml tubes,16 x 0.5 ml tubes or microtiter plates in a 5 x 5 grid format. The Eppendorf Mastercycler® Personal is an essential instrument of our lab. For the last six years, it is being used for regular PCR of the DNA for cloning purpose. Apart from our lab, it is available to everyone in the institute for the use. The various reagents and buffers required for the PCR reactions have to be brought by the person using it. |
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Location: |
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Department of Chemistry, |
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Contact: |
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Prof. Gurunath R. |
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Figure 1 |